Cloned (Comment) | Organism |
---|---|
gene XCV2729, belongs to operon xehypBA2-AA containing genes XCV2724, XCV2728, and XCV2729, DNA and amino acid sequence determination and analysis, and genetic structure. The promoter region of the operon (xehypBA2 and xehypAA) contains the plant-inducible promoter box for binding the regulator protein HrpX involved in pathogenicity, recombinant expression of wild-type and mutant enzymes without the signal peptide and fused with the trigger factor chaperone in Escherichia coli strain BL21(DE3), recombinant expression of the operon in transgenic Solanum lycopersicum strain Micro-Tom | Xanthomonas euvesicatoria |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a single-gene deletion mutant, DELTAxehypBA2 and a triple-gene deletion mutant DELTAxehypBA1-BA2-AA. The mutants remain pathogenic, and mutations of the operon or the single genes XCV2724, XCV2728, and XCV2729 have no effect on nonhost resistance, either, indicating that these three enzymes are not involved in either pathogenicity or nonhost resistance reactions | Xanthomonas euvesicatoria |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme contains a 42 amino-acid signal peptide | Xanthomonas euvesicatoria | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas euvesicatoria | Q3BS03 | - |
- |
Xanthomonas euvesicatoria 85-10 | Q3BS03 | - |
- |
Xanthomonas euvesicatoria UPB139 | Q3BS03 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O | - |
Xanthomonas euvesicatoria | 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose | - |
? | |
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O | - |
Xanthomonas euvesicatoria 85-10 | 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose | - |
? | |
4-O-(beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + H2O | - |
Xanthomonas euvesicatoria UPB139 | 4-O-(beta-L-arabinofuranosyl)-(2S,4S)-4-hydroxyproline + beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose | - |
? | |
additional information | substrate specificity, overview. Enzyme XeHypBA2 releases the disaccharide Araf-beta1,2-Araf from Araf-beta1,2-Araf-beta1,2-Araf-beta-Hyp (Ara3-Hyp) | Xanthomonas euvesicatoria | ? | - |
? | |
additional information | substrate specificity, overview. Enzyme XeHypBA2 releases the disaccharide Araf-beta1,2-Araf from Araf-beta1,2-Araf-beta1,2-Araf-beta-Hyp (Ara3-Hyp) | Xanthomonas euvesicatoria 85-10 | ? | - |
? | |
additional information | substrate specificity, overview. Enzyme XeHypBA2 releases the disaccharide Araf-beta1,2-Araf from Araf-beta1,2-Araf-beta1,2-Araf-beta-Hyp (Ara3-Hyp) | Xanthomonas euvesicatoria UPB139 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 152000, recombinant untagged enzyme, SDS-PAGE | Xanthomonas euvesicatoria |
Synonyms | Comment | Organism |
---|---|---|
GH 121 beta-L-arabinofuranosidase | - |
Xanthomonas euvesicatoria |
GH121 beta-L-arabinobiosidase | - |
Xanthomonas euvesicatoria |
xcv2729 | - |
Xanthomonas euvesicatoria |
XeHypBA2 | - |
Xanthomonas euvesicatoria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Xanthomonas euvesicatoria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Xanthomonas euvesicatoria |
Organism | Comment | Expression |
---|---|---|
Xanthomonas euvesicatoria | the operon is upregulated by transcription factor HrpX | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 121, GH121 | Xanthomonas euvesicatoria |
malfunction | single deletion mutants of genes XCV2724, XCV2728, and XCV2729 and the triple deletion mutant remain pathogenic, and mutations of the operon or the single genes have no effect on nonhost resistance, indicating that the enzyme is not involved in either pathogenicity or nonhost resistance reactions | Xanthomonas euvesicatoria |
physiological function | enzymes XCV2724, XCV2728, and XCV2729 in Xanthomonas euvesicatoria degrade the arabinofuranooligosaccharides present on hydroxyproline (Hyp)-rich glycoproteins (HRGPs) such as extensin and solanaceous lectins in plant cell walls. These enzymes work coordinately to degrade the oligosaccharides. The main oligosaccharide structure of Ara4-Hyp on the HRGPs is degraded to Ara3-Hyp, then to Ara-Hyp, and finally to Ara monosaccharides by the action of these three enzymes. Structure of arabino-oligosaccharides on extensin and solanaceous lectins in plant cell walls, overview. HRGPs containing oligosaccharide substrates have been reported to contribute to plant defense. The enzyme are not involved in either pathogenicity or nonhost resistance reactions | Xanthomonas euvesicatoria |